The role of phytoestrogens as endocrine disrupting agents is of emerging relevance in reproduction, as transgenerational impacts continue to be uncovered. To investigate whether oestrogen-like compound equol could impact reproduction over subsequent generations, a drosophila melanogaster model was used. Male and female flies (P1) were exposed from hatch to a period of peak fertility, to a physiologically relevant level of dietary equol (5mm). P1 were then either mated to a non-exposed partner of the same age, or an exposed partner. The subsequent offspring (F1) were then mated to either a partner from exposed or non-exposed parents. All matings were done in replicates of 10. The seminal vesicles of 5 males per group, per generation were also measured. In the P1 generation, exposure to equol reduced egg-producing capacity from both males and females regardless of whether they were mated to a control or exposed partner (control 31.25 ± 2.9 eggs per lay vs exposed female 20.27 ± 2.7 vs exposed male 15.08 ± 2.3, P=0.006). The number of subsequent offspring (F1) was also reduced (control 16 ±3.1 offspring per mating vs exposed P1 female 5.6 ± 0.3 vs exposed P1 male 2.5 ± 0.5, P<0.001). F1 flies with either a male or female equol-exposed parent had reduced egg-producing capacity (control 48.5 offspring per lay, vs 2.6 exposed female P1, 13.33 exposed male P1, P =0.015). Male F1 had reduced seminal vesicle size if either parent had been exposed to equol (control 0.0771 ± 0.002 mm2, vs 0.057 ± 0.001 mm2 from exposed female parent, 0.066 ± 0.001 mm2 from exposed male parent, P =0.015). Male F2 had reduced seminal vesicle size if exposure occured on the maternal side (P= 0.006). Further work is warranted to determine how equol is able to program the reproductive capacity of exposed individuals and their descendants.