Understanding orchestration of human embryo and endometrium during early implantation helps to improve the outcome of pregnancy. At the beginning of implantation, competent embryos can modify endometrium through secreted paracrine signals. Apical surface of endometrial epithelial cells (EECs) is the first part on the maternal side interacting with embryo during implantation. Using a human embryonic stem cells derived trophoblastic spheroids (BAP-EB), we previously showed that antibody blocking of E-cadherin on the surface of receptive Ishikawa cell line significantly reduced BAP-EB attachment rates. It was reported that p120-catenin is essential for E-cadherin stability. In this study, we further examined the roles of p120-catenin and E-cadherin during early implantation process. Using live-cell immunofluorescent staining, we confirmed the expression of E-cadherin on the apical surface of Ishikawa cell line and primary EEC isolated from human endometrial aspirates at 7/8 days after luteinizing hormone surge (LH+7/8). We further found that conditioned media (CM) collected from attachment competent BAP-EB differentiated for 72h significantly induced the expressions of membranous bound p120-catenin when compared to CM collected from attachment incompetent BAP-EB differentiated for 48h. Furthermore, knockdown of p120-catenin in Ishikawa cells by siRNA transfection significantly reduced E-cadherin expression in Ishikawa cells. Concordantly, p120-catenin knockdown also significantly reduced the BAP-EB attachment rates and spreading areas. Most importantly, immunoblotting results of primary EEC collected at LH+7/8 day showed that the protein levels of E-cadherin and p120-catenin were significantly higher in EEC collected from women who ultimately gave livebirth (n=27) when compared to those collected from women who were not pregnant (n=35). Our results suggest that the competent embryo might induce the expressions of endometrial p120-catenin which maintain the apical expression of E-cadherin during implantation process. The current data further prompt to the use of p120-catenin and E-cadherin as potential receptivity markers for predicting pregnancy outcomes.