In Assisted Reproductive Technology (ART), oocyte retrieval is a vital step. It was initially performed through laparoscopy, which was complex and difficult and of low efficiency. Ultrasound-guided transvaginal oocyte retrieval was safer and more effective; it is presently the standard operation for in vitro fertilization (IVF) treatment. Theoretically, oocyte retention is achievable after the initial aspirate due to abnormal development of the follicle or oocyte and human technical factors, and such retention could be overcome by recurrent follicular flushing. Follicular flushing is considered to maximize the number of oocytes retrieved and thereafter to improve the rate of IVF pregnancy. There are a number of factors that can affect egg collection and/or egg damage. These involve variables like pump vacuum flow, velocity; needle bore size and length, follicle pressure and size, and collection techniques. Cook Medical Technology, Brisbane, has developed appropriate equipment to study the factors influencing the success of egg collection and the cause of egg trauma. Experimental & physical aspects of oocyte retrieval Apart from a comparison between manual and mechanical suction on the impact of zonal damage , surprisingly little has been reported on oocyte collection theory until the studies carried out by Cook Medical Technology, Brisbane . A variety of variables may impact oocyte collection and/or ovary damage. These include variables like vacuum flow of the pump, velocity, size and length of the needle lumen, follicular pressure and size and collection techniques. Cook Medical Technology, Brisbane, established appropriate equipment for the study of factors affecting the performance of oocyte collection and the cause of oocyte trauma . Complications The ultrasound-guided transvaginal technique is a very efficient and simple procedure. However, this should not distract from the fact that a number of potentially dangerous complications exist, consisting mainly of hemorrhage, trauma to pelvic anatomical structures, and infection